Journal: Frontiers in Immunology

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

doi: 10.3389/fimmu.2024.1383932

Figure Lengend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Article Snippet: Fibronectin , REAL555 , 50 , 130-122-864 , PE , Miltenyi Biotec.

Techniques: Imaging

Journal: European journal of histochemistry : EJH

Article Title: Sodium hyaluronate promotes proliferation, autophagy, and migration of corneal epithelial cells by downregulating miR-18a in the course of corneal epithelial injury.

doi: 10.4081/ejh.2023.3663

Figure Lengend Snippet: Figure 5. SH induced autophagy and cell migration by downregulating miR-18a in CEI model cells. CEI model cells were treated with SH and miR-18a mimics, respectively. A) Cell migration was analyzed by the Transwell assay, and the numbers of migrated cells in each group were counted. B) Western blotting showed changes in LC3B, Beclin1, and P62 expression. C) LC3B expression was examined by IF staining. Magnification: x200.

Article Snippet: The primary antibodies used were Col1A1 (BA0325, 1:800; Boster), FN (A00564-1, 1:1000; Boster), LC3B (ab192890, 1:2000; Abcam), Beclin1 (BA3123-2, 1:1500; Boster), P62 (BA2849, 1:1000; Boster), and GAPDH (ab8245, 1:5000; Abcam).

Techniques: Migration, Transwell Assay, Western Blot, Expressing, Staining

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Dioscin ameliorates peritoneal fibrosis by inhibiting epithelial-to-mesenchymal transition of human peritoneal mesothelial cells via the TLR4/MyD88/NF-κB signaling pathway

doi:

Figure Lengend Snippet: Dioscin inhibits LPS-induced fibrosis in HMrSV5 cells. Western blotting for detecting protein levels of α-SMA, collagen I and fibronectin. ###P < 0.001 compared with negative control group or dioscin (1.0 μg/ml) group. ***P < 0.001 compared with LPS group. NC, negative control; LPS, lipopolysaccharide; Dio, Dioscin; α-SMA, α-smooth muscle actin.

Article Snippet: Then the membranes were incubated with following primary antibodies: α-SMA (1:100, Proteintech Group), collagen I (1:100, Proteintech Group), fibronectin (1:250, Abcam), TLR4 (1:1000, Proteintech Group), MyD88 (1:1000, Proteintech Group), NF-κB (1:1000, Proteintech Group), TGF-β1 (1:1000, Proteintech Group), p-Smad2/Smad2 (1:1000, Proteintech Group), respectively.

Techniques: Western Blot, Negative Control

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Dioscin ameliorates peritoneal fibrosis by inhibiting epithelial-to-mesenchymal transition of human peritoneal mesothelial cells via the TLR4/MyD88/NF-κB signaling pathway

doi:

Figure Lengend Snippet: Dioscin inhibits EMT and fibrosis through TLR4/MyD88/NF-κB pathway in HMrSV5 cells. A. Western blotting for assessing protein levels of TLR4, MyD88, NF-κB, TGF-β1, p-Smad2, Smad2, α-SMA, collagen I and fibronectin. B. immunofluorescence assay for detecting expressions of α-SMA, collagen I and fibronectin in LPS+TLR4 group and LPS+TLR4+dioscin (0.5 μg/ml) group. ###P < 0.001 compared with negative control group or Ppicza group. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with LPS+ pPICZA group or LPS+TLR4 group. NC, negative control; LPS, lipopolysaccharide; Dio, Dioscin; α-SMA, α-smooth muscle actin; MyD88, myeloid differentiation factor 88; NF-κB, nuclear factor κB; TGF-β1, transforming growth factor-β1; TLR4, Toll-like receptor (TLR) 4.

Article Snippet: Then the membranes were incubated with following primary antibodies: α-SMA (1:100, Proteintech Group), collagen I (1:100, Proteintech Group), fibronectin (1:250, Abcam), TLR4 (1:1000, Proteintech Group), MyD88 (1:1000, Proteintech Group), NF-κB (1:1000, Proteintech Group), TGF-β1 (1:1000, Proteintech Group), p-Smad2/Smad2 (1:1000, Proteintech Group), respectively.

Techniques: Western Blot, Immunofluorescence, Negative Control

Journal: Experimental eye research

Article Title: Sympathetic activation leads to Schlemm's canal expansion via increasing vasoactive intestinal polypeptide secretion from trabecular meshwork.

doi: 10.1016/j.exer.2022.109235

Figure Lengend Snippet: Figure 1. Authentication of primary cultured rat trabecular meshwork (TM) cells. (A) Isolation and proliferation of primary TM cells observed under light microscope. a. Tissue scraps cut from corneoscleral rims. Co: cornea; Ir: iris. Blue triangles indicate the location where TM is supposed to reside. b. Primary TM cells started to crawl out of tissue scraps (indi cated by *) and adhere (indicated by black triangles). c. The attached cells gradually formed “clusters” and became contact-inhibited. d-f. Passage 3 TM cells presented typical spindle-like “fibroblast” phenotype and became cobblestone-like once confluent. Scale bars = 100 μm. (B) Immunofluorescence staining of cultured TM cells with anti-fibronectin, anti-laminin, anti-neuron-specific enolase (NSE), anti-factor- Ⅷ-related antigen (FⅧRAg) antibodies. Scale bar = 100 μm. (C–E) Cells were treated with 200 nM dexamethasone (Dex) for 7 days. Immunohistochem istry staining showed that over fifty percent of TM cells were myocilin-positive (the whole cell was yellow-stained) and was statistically different to that of vehicle control. Western blotting with anti- myocilin antibody showed a doublet at 55 kDa for the Dex-treated group. MYOC: myocilin. Scale bar = 300 μm. (F) Cells were treated with 100 nM Dex or ethanol control for 7 days and dyed with phalloidin. Cross-linked actin networks (CLANs) were observed and indicated by white triangles. Scale bars = 100 μm.

Article Snippet: Expression of fibronectin (M00564-3, BOSTER, China, 1:50), laminin (A03522, BOSTER, China, 1:50), neuron-specific enolase D. Xu et al.

Techniques: Cell Culture, Isolation, Light Microscopy, Immunofluorescence, Staining, Control, Western Blot

Journal: European journal of histochemistry : EJH

Article Title: Sodium hyaluronate promotes proliferation, autophagy, and migration of corneal epithelial cells by downregulating miR-18a in the course of corneal epithelial injury.

doi: 10.4081/ejh.2023.3663

Figure Lengend Snippet: Figure 2. SH markedly downregulated miR-18a expression and mediated autophagy-related protein expression in mice with CEI. CEI model mice were established and then treated with SH. A) Changes in miR-18a expression in mice were identified by using qPCR at 0, 12, 24, 48, and 72 h. B) RT-qPCR analyses of CTGF, TGF-β, Col1A1, and FN expression. C) The concentrations of CTGF and TGF-β were detected with ELISA kits at 72 h post injury. D,E) Western blotting was used to monitor changes in Col1A1, FN, LC3B, Beclin 1, and P62 expression in corneal tissues at 72 h post injury.

Article Snippet: The primary antibodies used were Col1A1 (BA0325, 1:800; Boster), FN (A00564-1, 1:1000; Boster), LC3B (ab192890, 1:2000; Abcam), Beclin1 (BA3123-2, 1:1500; Boster), P62 (BA2849, 1:1000; Boster), and GAPDH (ab8245, 1:5000; Abcam).

Techniques: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Western Blot